New full-time Cambridge head coach Mark Bonner has injury concerns over Samir Carruthers and Paul Lewis for the visit of Cheltenham.
The midfield duo have missed training this week and will face late fitness checks before Bonner names his squad.
Forward Harvey Knibbs is closing in on a return but will not be rushed back into action.
Midfielder Luke Hannant is making good progress in his recovery but is expected to remain out for a few more weeks.
Cheltenham captain Ben Tozer is facing up to six weeks on the sidelines with an ankle injury.
Tozer, who damaged ligaments in the win at Colchester and missed last weekend’s 0-0 draw with Port Vale, underwent a scan to reveal the extent of the damage.
Striker Luke Varney is also expected to miss several weeks of action after suffering a hamstring injury at Colchester.
Manager Michael Duff expects striker Jonte Smith to rejoin the squad on Friday after being away on international duty with Bermuda.