MSPs have questioned a decision to schedule the Dons semi-final away game at Hampden too early in the afternoon.
Kick-off for the game against Greenock Morton is set for 12.15pm on Saturday, October 22.
But MSPs have complained fans will struggle to get to the game as there is no direct train service between Aberdeen and Glasgow due to track repairs.
Moray MSP Richard Lochhead has lodged a motion at Holyrood calling on the Scottish Professional Football League (SPFL) to kick-off later that day, supported by North-east MSP Lewis Macdonald.
He said: “The current kick-off time could prevent many fans from the north of Scotland from making the trip.
“I call on the SPFL to listen to the travelling supporters, who it considers the lifeblood of Scottish football clubs, and reconsider the scheduling of this Scottish League Cup tie.”
Aberdeen Central MSP Kevin Stewart has also written to the SPFL chief executive, urging him to reconsider.
He said: “It’s unbelievable such total disregard has been shown to supporters from Aberdeen who will want to make it down for a game they will be very excited to see.
“This is a semi-final, for goodness sake, and due consideration should have been given to the supporters from both teams playing.
“Instead we are seeing a major advantage being handed to supporters based much closer to the Glasgow venue.”
Mr Stewart said fans’ needs should not be put behind those of sponsors.
He said: “It looks as though all this is being done to appease the broadcaster BT Sport, which flies in the face of football -the beautiful game – being for everyone.
“I sincerely hope the SPFL have a satisfactory response for a decision that has put so much unrest and inconvenience to Aberdeen supporters.
“A semi-final is supposed to be a great day out for a team’s supporters but will now no doubt be a rushed 150 mile trip down to Glasgow for those that can still manage.”
The SPFL declined to comment.